Genalice 96A Automated Viral RNA Isolation Kit

1. Based on Dynal magnetic beads-based technology.
2. Automated isolation and purification of RNA from biological samples.
3. The product is intended to be used by professional users that are trained in molecular
   biological techniques.

Test Principles

⚫ The 96A Automated Viral RNA Isolation Kit is intended to be used in combination
with KingFisher™ Flex Purification System or Auto-Pure96 Nucleic Acid
Purification System.

The kit provides reagents for fully automated purification of
RNA. Magnetic beads-based technology enables purification of high-quality nucleic
acids that are free of nuclease, proteins and other impurities.

The purified nucleic
acids are ready for the downstream applications such as RT-qPCR. KingFisher™
Flex Purification System or Auto-Pure96 Nucleic Acid Purification System all the
steps of purification procedure.

Up to 96 samples are processed in a single run.

The purification contains the procedures as below that ensure the safe and
reproducible handling of potential infectious samples.

⚫ Lysis & Bind
⚫ Wash
⚫ Elution

Materials Provided

The 96A Automated Viral RNA Isolation Kit contents are listed in Table 1.
Table 1: Contents of the 96A Automated Viral RNA Isolation Kit

When RNA extraction is performed using the 96A Automated Viral RNA Isolation Kit on
the KingFisher™ Flex Purification System.
1. Ensure that the KingFisher™ Flex Purification System is set up for processing.
2. Ensure that the“ 3DMed-N96” program has been set up.
Note:The program “3DMed-N96”for KingFisher™ Flex is Provided by 3DMed.
3. Transfer the inactivated clinical sampling tubes to room temperature. Vortex the
tubes for 10 seconds.
4. Transfer Proteinase K to room temperature and mix it well for 5 times up and down.
5. Check when the crystals or turbidness has appeared in Lysis buffer plate, heat it at
50°C until it has been dissolved, and centrifugate the plate at 2,000 rpm for
seconds.
6. Peel the plate sealing film from Lysis buffer plate carefully, add 200µL inactivated
sample and 20µL Proteinase K into each of the wells in the plate.
Important Note: if an Internal Control is required for downstream application, mix the required
Internal Control (provided from the downstream application) with 200μL of sample and 20μL of
Proteinase K.
7. Gently load the 96 Tip comb in the Wash buffer plate.
8. Peel the plate sealing film of Wash buffer plate and Elution buffer plate carefully.
9. Switch on the KingFisher™ Flex Purification System, and select “3DMed-N96”
program.
10. Load the prepared plates into the corresponding position when start the program.
11. Immediately remove the Elution buffer plate from the instrument and pipette the
RNA into PCR plate for next step, or seal the Elution buffer plate by the sealing
film and storage at 2-8℃ for 24 hours.
12. Discard all the used 96-well deep plates and 96 Tip comb to the fixed area

Protocol

1. Transfer the inactivated clinical sampling tubes to room temperature.
2. Transfer Proteinase K to room temperature and mix it well for 5 times up and down.
3. Check when the crystals or turbidness has appeared in Lysis buffer plate, heat it at
50°C until it has been dissolved, and centrifuge the plate at 2,000 rpm for seconds.
4. Peel the plate sealing film from Lysis buffer plate carefully, add 200µL inactivated
sample and 20µL Proteinase K into each of the wells in the plate.
Important Note: if an Internal Control is required for downstream application, mix the required Internal Control (provided from the
downstream application) with 200μL of sample and 20μL of Proteinase K.
4. Gently load the 96 Tip comb in the Wash buffer plate.
5. Switch on the KingFisher™ Flex Purification System, and select “3DMed-N96” program.
6. Peel the plate sealing film of Wash buffer plate and Elution buffer plate carefully.
7. Load the prepared plates into the corresponding position when start the program.

Otrher technical documents about 3DMed Products:

1. 96A(Extraction free) IFU
2. 96A(Extraction free) SOP
3. 96A(Extraction free) pre-setting extraction protocol on the KingFisher Flex extractor
4. 3DMed Fast Detection Kit(V5.1) SOP
5. Omicron Detection Kit Brochure
6. Omicron Detection Kit IFU