H3K4 Me3 Antibody

Product name: 

TriMethyl-Histone H3-K4 Rabbit pAb

Catalog No.       

A2357

Host species:    

Rabbit

Purification method:     

Affinity purification

Isotype:

IgG

Form:

Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Swiss Prot:         

Q16695

Gene ID:

8290

Uni-prot:

Q16695

Immunogen:     

A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Reactivity:         

Human, Mouse, Rat, Other (Wide Range)

Storage buffer:

Store at -20℃. Avoid freeze / thaw cycles.

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Applications:    

ChIP, IF, IHC, IP, WB

Positive samples:           

HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein

Cellular location:            

Chromosome, Nucleus

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.